Mouse MIP-1 beta (Macrophage Inflammatory Protein 1 Beta) ELISA Kit (MOES00629)

Designed for the accurate measurement of MIP-1 beta levels in mouse serum, plasma, and cell culture supernatants, this Sandwich ELISA kit from Assay Genie uses a colorimetric detection method and delivers reproducible results for inflammatory research. MIP-1 beta is a key cytokine involved in the inflammatory response, with dysregulation implicated in various inflammatory diseases and conditions, making it a valuable biomarker for studying immune cell recruitment and activation.

The assay employs pre-coated microplate wells, a biotinylated detection antibody, and an Avidin-Horseradish Peroxidase (HRP) conjugate, with absorbance read at 450 nm ± 2 nm. Reported sensitivity is 9.38 pg/mL, detection range 15.63-1000 pg/mL, and recovery 80%-120%. The protocol requires a sample volume of 100 μL and runs in 3 h 30 min. The kit recognizes Mouse MIP-1β, with no significant cross-reactivity or interference between Mouse MIP-1β and analogues observed.

Validated recovery for EDTA Plasma (n=8) ranges 94-108% with an average of 100%, and for Cell Culture Media (n=8) ranges 95-110% with an average of 100%. Linearity has been confirmed across 1:2 to 1:16 dilutions for serum, EDTA plasma, and cell culture media. Intra-assay CV is reported between 4.91% and 6.67%, and inter-assay CV between 6.27% and 7.49%.