Mouse MIP-1 alpha/CCL3 ELISA Kit

Assay Genie's colorimetric sandwich ELISA, configured with a double-antibody format, is designed for the precise quantitative measurement of MIP-1 alpha (CCL3) in mouse samples. The kit is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, supporting research into this chemokine's roles in inflammatory responses, immune regulation, and cell recruitment.

Reported analytical performance includes a sensitivity of 9.375 pg/mL and a standard range of 15.625–1000 pg/mL. Linearity has been evaluated across serum, EDTA plasma, and heparin plasma at 1:2, 1:4, and 1:8 dilutions, with recovery ranges documented for the same sample types. The assay uses a biotin-labeled detection antibody with HRP-Streptavidin (SABC) and TMB substrate, with absorbance read at 450 nm, and kit components are intended for storage at 2-8°C for 12 months.