Mouse MIF (Macrophage Migration Inhibitory Factor) ELISA Kit (MOES01252)

Assay Genie offers a colorimetric sandwich ELISA for the quantification of mouse MIF (macrophage migration inhibitory factor). MIF is described as a key cytokine involved in the regulation of innate and adaptive immune responses, as well as inflammation and tissue repair processes. The kit measures MIF in serum, plasma, tissue lysates and other biological fluids using a sample volume of 100 μL, with a reported assay time of 3 h 30 min, and is listed for immunology and cancer research applications.

Reported specifications include a sensitivity of 9.38 pg/mL and a detection range of 15.63-1000 pg/mL, with stated recovery of 80%-120%. The kit recognises mouse MIF, and the manufacturer reports no significant cross-reactivity or interference between mouse MIF and analogues. In recovery testing, EDTA plasma showed an average recovery of 93% (range 88-100%) and cell culture media an average of 96% (range 89-105%).

Precision data list intra-assay CV values of 4.47%, 6.57% and 6.8%, and inter-assay CV values of 6.51%, 7.01% and 7.83%. Linearity was assessed in serum, EDTA plasma and cell culture media at dilutions from 1:2 to 1:16, with reported averages at the 1:2 dilution of 101% for serum, 104% for EDTA plasma and 94% for cell culture media.