Mouse Melanin-concentrating hormone receptor 1 (Mchr1) ELISA Kit

A colorimetric sandwich ELISA from Assay Genie designed for the quantitative detection of mouse Melanin-concentrating hormone receptor 1 (MCHR1). The kit is intended for use with mouse serum, plasma, tissue homogenates, cell culture supernates and other biological fluids, with reactivity confirmed for mouse samples and specificity toward natural and recombinant mouse MCHR1.

According to the manufacturer specification, the assay covers a detection range of 0.78-50 ng/mL with a sensitivity of 0.393 ng/mL. The protocol is a standard sandwich ELISA workflow using a microplate reader set to 450 nm. Component storage conditions vary, with reagents such as standards, diluents and detection reagents stored at -20°C and wash buffer, substrate and stop solution stored at 4°C.

MCHR1 is a G-protein coupled receptor for melanin-concentrating hormone, coupled to G proteins that inhibit adenylyl cyclase and to G proteins that activate phosphoinositide hydrolysis. It is implicated in appetite regulation, energy homeostasis and related metabolic and neurological research. The product is supplied for research use only.