Mouse Mdm2 p53 Binding Protein Homolog (MDM2) ELISA Kit

MDM2, the murine homolog of the human Mdm2 oncoprotein, is a RING finger-type E3 ubiquitin ligase that binds the transactivation domain of p53 and targets p53 for proteasomal degradation, making its quantitative measurement central to studies of the p53 tumor-suppressor axis, the DNA damage response, and oncogene-driven proliferation. This immunoassay kit from Assay Genie is configured for quantitative detection of mouse MDM2 in research samples and is validated for reactivity against murine tissue. The microplate-based workflow supports 96 individual determinations in a single plate format and is appropriate for cell culture-derived samples and tissue homogenates or cell lysates prepared from excised mouse tissues. Each kit includes the reagents needed to run the full assay protocol — pre-coated plate, standard, detection antibody, conjugate, wash buffer, substrate, and stop solution — to produce an absorbance readout compatible with a standard microplate reader setup.