Mouse MCP-1 PharmaGenie ELISA Kit (SBRS1464)

The Mouse MCP-1 PharmaGenie ELISA Kit from Assay Genie quantitatively measures monocyte chemoattractant protein-1 (MCP-1/CCL2), a key inflammatory chemokine secreted by endothelial cells, monocytes, and macrophages in response to cytokine stimulation. Researchers deploy this microplate-based assay in inflammation, atherosclerosis, obesity, and metabolic disease studies to track monocyte recruitment signals in murine models.

The pre-coated plate uses anti-MCP-1 capture antibodies and delivers a detection range of 15.6 to 1000 pg/mL, with a sensitivity below 2 pg/mL. Each reaction requires 100 uL of sample, and the protocol is compatible with cell culture supernatants, plasma, serum, cell lysates, and tissue lysates. Reagent handling involves equilibrating components to room temperature before use; the kit should be stored refrigerated at 4°C, protected from light, with standard freeze-thaw cycle limitations.

The standard curve is generated using recombinant mouse MCP-1, and the assay workflow uses streptavidin-HRP detection. Each kit provides sufficient reagents for one microplate run, including coated wells, biotinylated detection antibody, standards, and TMB substrate solution, for research use only.