Mouse Mbl2/ Mannose-binding protein C ELISA Kit

From Assay Genie, this Sandwich ELISA kit provides quantitative measurement of mouse MBL2 (Mannose-Binding Protein C) using a double antibody format and colorimetric detection. MBL2 is a vital component of the innate immune system, playing a critical role in recognizing and neutralizing pathogens through the lectin pathway, and its dysregulation has been implicated in various infectious diseases, autoimmune disorders, and inflammatory conditions, underscoring its importance as a biomarker and therapeutic target in immunological research.

The kit is reactive with mouse samples and accepts serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Sensitivity is 46.875 pg/mL with a working range of 78.125-5000 pg/mL. Storage is at 2-8°C for 12 months. The associated Uniprot entry is P41317, and the research area is immunology.

Linearity across dilutions (1:2, 1:4, 1:8) is reported as 86-101% / 85-97% / 81-99% for serum (n=5), 90-99% / 85-101% / 83-94% for EDTA plasma (n=5), and 87-102% / 82-95% / 91-97% for heparin plasma (n=5). Recovery ranges are 85-105% (average 94%) for serum, 92-98% (average 96%) for EDTA plasma, and 85-100% (average 97%) for heparin plasma. Detection uses a biotin-labeled antibody followed by HRP-Streptavidin (SABC) and TMB substrate, with absorbance measured at 450 nm.