Mouse MBL-1 PharmaGenie ELISA Kit (SBRS1461)

Quantitative measurement of mouse mannose-binding lectin supports research into the lectin pathway of complement activation, and the MBL-1 ELISA Kit from Assay Genie is built around that biomarker for immunology, infectious disease, and pharmacology studies. In murine systems, the MBL-1 paralogues act as soluble pattern-recognition molecules secreted by the liver, with documented involvement in innate immune defense, ischemic tissue injury, sepsis outcomes, and complement-driven inflammation. Tracking circulating MBL-1 therefore serves as a useful readout in studies of vaccine adjuvanticity, metabolic disease, and in vivo challenge models.

Configured as a multi-well plate assay, the kit is supplied as a complete pack with pre-coated microwells, detection reagents, a calibrator series, and the buffers required to run the full plate in a single experiment. The assay accepts cell culture supernatants alongside circulating samples from mice, supporting both direct profiling of clinical-type specimens and conditioned-medium work from primary cells or tissue explants. It is intended for research use only and fits standard microplate absorbance readers.

Workflow involves diluting standards and samples, loading them onto the plate, incubating, washing, adding the detection reagent, and reading absorbance to quantitate results against the standard curve generated from the calibrator series. Reagents should be stored refrigerated between uses to preserve antibody binding activity, and the standard should be aliquoted into single-use vials to avoid repeated freeze-thaw cycles that erode performance.

Academic groups and contract research organizations working on complement biology, infection models, and inflammation research use this format for moderate-throughput screening campaigns. The supplied pack also suits retrospective studies on banked mouse samples, where longitudinal MBL-1 measurements can be correlated with disease progression or treatment response in cohort-based experimental designs.