Mouse Matrix Metalloproteinase 2 (MMP2) ELISA Kit

MMP2, the 72 kDa zinc-dependent gelatinase also known as Gelatinase A or type IV collagenase, sits at the center of extracellular matrix remodeling in murine models of cancer metastasis, cardiac injury, pulmonary and hepatic fibrosis, and inflammatory disease. Assay Genie's Mouse Matrix Metalloproteinase 2 (MMP2) ELISA Kit provides quantitative measurement of this enzyme in serum samples, plasma samples, and clarified tissue homogenates from mouse, supporting research use only in academic, pharmaceutical, and biotechnology laboratories.

The kit ships with pre-coated microplate wells, calibration standards, biotinylated detection antibody, and assay buffers sufficient for 96 determinations, providing enough material for a full standard curve and replicate analyses of experimental samples. Because MMP2 circulates as both a latent pro-form and a cleaved active enzyme, some investigators pre-treat specimens with activating agents such as APMA to discriminate between the two, and parallel gelatin zymography is frequently run alongside ELISA readouts as orthogonal confirmation of enzymatic activity. Unopened components should be refrigerated and equilibrated to room temperature before use, and the assay is intended strictly for laboratory research, not for diagnostic or therapeutic decision-making.