Mouse Mast Cell Protease-6/Mcpt6 PharmaGenie ELISA Kit (SBRS1468)

Assay Genie provides a quantitative ELISA kit for measuring mouse Mast Cell Protease-6 (Mcpt6), a chymase-family serine protease secreted predominantly by mucosal mast cells. Researchers studying intestinal inflammation, helminth infection, allergic disease, and mast cell degranulation use this kit to quantify Mcpt6 release in mouse cell culture supernatant, plasma, and serum samples.

The immunoassay uses a pre-coated microplate, lyophilized recombinant standards, a biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, chromogenic substrate, and stop solution, sufficient for 96 individual determinations. During the workflow, capture antibody bound to the plate immobilises Mcpt6 from standards and samples; after washing, the detection antibody is added, followed by streptavidin-HRP and a chromogenic substrate whose absorbance is read on a standard plate reader. The kit is configured for specific detection of mouse Mcpt6.

Standards are calibrated to permit interpolation of unknown sample concentrations from a generated standard curve. Reagents should be stored refrigerated, and reconstituted standards should be aliquoted and frozen to limit repeated freeze-thaw cycles. Intended for research use only.