Mouse Map2k7 ELISA Kit

From Assay Genie, this Sandwich ELISA kit is designed for the quantitative detection of MAP2K7 in mouse samples, using a double-antibody format and colorimetric detection. The assay targets MAP2K7 (mitogen-activated protein kinase kinase 7), a key regulator of cellular signaling pathways involved in cell growth, differentiation, and survival. The protein is also referred to as MAPKK 7, MEK 7, JNK-activating kinase 2, MAPK/ERK kinase 7, c-Jun N-terminal kinase kinase 2, and JNK kinase (UniProt Q8CE90; KEGG K04431; GeneID 26400). Dysregulation of MAP2K7 has been implicated in cancer, inflammation, and autoimmune disorders, supporting its use as a research target in cell death studies.

Reported linearity spans 82–102% across serum, EDTA plasma, and heparin plasma at 1:2, 1:4, and 1:8 dilutions, with spike-and-recovery averages of 92% in serum, 97% in EDTA plasma, and 99% in heparin plasma. The procedure uses a biotin-labeled detection antibody, HRP-streptavidin (SABC), and TMB substrate, with absorbance read at 450 nm. Components are stored at 2–8°C for up to 12 months.