Mouse Macrophage metalloelastase (Mmp12) ELISA Kit

Designed for the precise quantification of Macrophage metalloelastase (MMP12) in mouse samples, this Sandwich ELISA Kit from Assay Genie enables measurement in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. The assay detects natural and recombinant mouse MMP12 and is configured as a colorimetric ELISA with a stated detection range of 15.6–1000 pg/mL and a sensitivity of 7.83 pg/mL.

MMP12 is a key extracellular matrix-degrading enzyme implicated in tissue remodeling and inflammation, and according to the manufacturer its dysregulation has been associated with chronic obstructive pulmonary disease (COPD), emphysema, and atherosclerosis, supporting its use as a biomarker for these conditions. Reported precision is 6.9% intra-assay CV and 8.6% inter-assay CV. Manufacturer linearity data across 1:2 to 1:16 dilutions span 84–119% for serum, 85–119% for EDTA plasma, and 93–112% for heparin plasma, with average recovery of 87% for serum and 89% for plasma.

For research use only, with cancer listed as the primary research area. The protein is described as a secreted protease of the peptidase M10A family with significant elastolytic activity, a preference for leucine at the P1′ site, and small hydrophobic residues (preferably alanine) occupying P3.