Mouse Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit

The Assay Genie immunoassay panel targets mouse macrophage inflammatory protein 3 alpha, the chemokine also designated CCL20, which signals through the CCR6 receptor to direct lymphocyte trafficking toward inflamed or mucosal surfaces. Investigators probing dermatitis, colitis, airway inflammation, lymphoid organ homing, or Th17 cell recruitment rely on quantitative measurement of this ligand in cell culture supernatants and tissue lysates to follow inflammatory kinetics across experimental conditions. Each kit ships with reagents supplied in individual vials sized for a full microplate workflow, and the colorimetric readout is captured on standard plate readers. Capture antibody arrives pre-coated onto the strip wells; prepared standards and experimental unknowns are dispensed in sequence, followed by a biotinylated detection reagent and streptavidin-HRP conjugate. Substrate conversion is terminated with an acidic stop solution before absorbance is recorded. The protocol calls for refrigerated storage of all components between uses to preserve binding activity through the full shelf life of the kit, supporting reproducible quantification across replicate wells and independent experiments.