Mouse Lysyl tRNA Synthetase (KARS) ELISA Kit

Quantitative measurement of mouse lysyl-tRNA synthetase (KARS) levels is enabled by this 96-assay ELISA kit from Assay Genie, formatted as a complete microplate with pre-prepared standards, detection reagents, and assay buffers. The enzyme charges tRNA with lysine during translation and is a research target in aminoacyl-tRNA synthetase biology, mitochondrial aminoacylation disorders, and inherited neuropathies such as Charcot-Marie-Tooth disease type 2D (CMT2D).

The protocol accepts microliter sample volumes and is validated for in vitro research use only. Storage guidance recommends refrigerating the unopened kit at 4 °C and protecting reagents from light to preserve activity through the labelled expiry date. Repeated freeze-thaw cycles of prepared standards should be avoided, and sample dilution is performed according to the supplied insert to maintain measurement linearity.

Investigators using mouse disease models, translational control studies, or tissue-specific expression profiling can apply the kit to compare wild-type and genetically modified cohorts, evaluate KARS abundance across different organs, and characterize enzyme expression under experimental perturbation.