Mouse Lysophosphatidylcholine Acyltransferase 4 (LPCAT4) ELISA Kit

Built on a two-antibody capture format, the Assay Genie Mouse LPCAT4 kit pairs a pre-immobilized capture antibody with a biotinylated detection antibody and a horseradish peroxidase (HRP)-streptavidin conjugate, using TMB as the chromogenic substrate. The standard curve is generated from a serial dilution series calibrated against recombinant mouse LPCAT4, allowing interpolation of unknown sample concentrations. The complete workflow typically proceeds over approximately 3.5 hours at room temperature, with washes performed between each incubation step using the supplied wash buffer concentrate. Researchers studying non-alcoholic fatty liver disease (NAFLD), arachidonic acid mobilization, or brain membrane lipid composition have applied this kit to mouse liver, brain, and adipose tissue lysates, where LPCAT4 transcript expression is most abundant. Each kit contains sufficient reagents for a single plate, including a sealed antibody-coated microplate, lyophilized recombinant standards, biotinylated detection antibody, HRP-streptavidin, TMB substrate, stop solution, wash buffer, and sample diluent. For best results, tissue homogenates should be clarified by centrifugation at 10,000 x g for 10 minutes at 4°C before loading, and freshly prepared samples are recommended over multiple freeze-thaw cycles to preserve LPCAT4 antigen integrity.