Mouse Lymphotoxin-alpha/Lta ELISA Kit

Assay Genie supplies a Sandwich ELISA built on a double-antibody format for the quantitative measurement of Lymphotoxin-alpha (LTA) in mouse samples. The assay uses a colorimetric detection method, with absorbance read at 450 nm after TMB substrate development. It is reactive with mouse analytes and is listed as compatible with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Reported performance figures include a sensitivity of 9.375 pg/mL and a working range of 15.625-1000 pg/mL. Linearity in serum (n = 5) is given as 85-103% at 1:2, 88-98% at 1:4, and 84-92% at 1:8; in EDTA plasma (n = 5) as 85-103%, 83-96%, and 86-96%; and in heparin plasma (n = 5) as 92-100%, 80-98%, and 90-104% across the same dilutions. Recovery ranges are 87-105% (average 92%) for serum, 85-99% (average 96%) for EDTA plasma, and 92-103% (average 95%) for heparin plasma. Reagents are stored at 2-8°C for 12 months.

According to the manufacturer's overview, Lymphotoxin-alpha is a key protein involved in the immune response, regulating inflammation and immune cell function, with dysregulation linked to inflammatory disorders and autoimmune diseases. The associated UniProt entry is P09225, and the protein is also referred to by synonyms including TNF-beta, Lta, tnfb, TNFSF1, TNFSF1B, LT, and LT-alpha.