Mouse LIFR (Leukemia Inhibitory Factor Receptor) ELISA Kit (MOES01238)

A quantitative immunoassay from Assay Genie measures leukemia inhibitory factor receptor (LIFR) concentrations in mouse-derived samples. LIFR is a transmembrane gp130-associated receptor that binds leukemia inhibitory factor (LIF) and propagates downstream JAK/STAT3 signaling, a cascade essential for maintaining mouse embryonic stem cell self-renewal and pluripotency.

Investigators working in stem cell biology, reproductive biology, neurodevelopment, and tumor microenvironment research apply this kit to characterize receptor abundance across various sample matrices, including sera, plasma preparations, tissue homogenates, and conditioned media from mouse model systems. Each kit supplies the reagents needed to run a complete plate of mouse LIFR measurements, with pre-coated wells, calibrated reference standards, and conjugate-based detection reagents.

The protocol uses an absorbance-based readout on a standard microplate spectrophotometer, with results calculated against the included standard curve. Reagents should be refrigerated upon arrival and protected from light to preserve conjugate activity. Unopened components remain stable through the printed expiration date when handled as directed. Single-use vials of standards and detection antibody minimize freeze-thaw cycles that could compromise binding kinetics. The kit is intended for research use only and supports in vitro investigations of the LIF/LIFR signaling axis in mouse tissues and cell culture models.