Mouse LIF/Leukemia inhibitory factor ELISA Kit

From Assay Genie, this sandwich ELISA kit is designed for the quantitative measurement of Leukemia Inhibitory Factor (LIF) in mouse samples. The assay uses a double-antibody format and is intended for research applications investigating this cytokine, which plays a role in cell differentiation, proliferation, and survival, with dysregulation linked to leukemia and inflammatory disorders.

The kit has a reported sensitivity of 46.875 pg/mL and a detection range of 78.125-5000 pg/mL. Compatible sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit should be stored at 2-8°C for 12 months.

Validation data from the manufacturer shows linearity in serum (85-104%, 94-101%, 80-98% at 1:2, 1:4 and 1:8 dilutions), EDTA plasma (85-94%, 88-101%, 82-99%) and heparin plasma (85-100%, 86-98%, 81-98%). Recovery ranges were 85-105% for serum (average 92%), 86-103% for EDTA plasma (average 95%), and 89-102% for heparin plasma (average 95%).