Mouse Leukotriene C4 synthase (Ltc4s) ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA Kit is intended for the quantitative detection of mouse Leukotriene C4 synthase (LTC4S) in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. The target enzyme catalyzes the conjugation of leukotriene A4 with reduced glutathione to form leukotriene C4, a potent mediator of inflammation and allergic reactions. Dysregulation of LTC4S has been associated with inflammatory conditions, making it a useful biomarker for studies of inflammatory pathways and potential therapeutic targets. The assay uses a colorimetric sandwich ELISA format with specificity for natural and recombinant mouse Leukotriene C4 synthase.

Reported performance includes a detection range of 15.6-1000 pg/ml, a sensitivity of 8.08 pg/mL, an intra-assay CV of 6.1% and an inter-assay CV of 8.8%. Recovery data is provided for serum and plasma, and linearity values are reported across dilutions for serum, EDTA plasma and heparin plasma matrices. The target protein is a homotrimer that interacts with ALOX5AP and ALOX5, and the kit falls within the Cardiovascular research area. Supplied for research use only.