Mouse Leukotriene B4 receptor 1/LTB4R ELISA Kit

From Assay Genie, this Sandwich ELISA kit is designed for the quantitative detection of mouse Leukotriene B4 receptor 1 (LTB4R) in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit uses a double antibody sandwich format with colorimetric detection and is intended for research into inflammation and immunity, where LTB4R acts as a key receptor in the inflammatory response.

Reported performance characteristics include a sensitivity of 0.188 ng/mL and a standard curve range of 0.313-20 ng/mL. Linearity across serum, EDTA plasma, and heparin plasma (n=5) at 1:2, 1:4, and 1:8 dilutions gave ranges of 91-104% / 95-99% / 90-100% for serum, 89-102% / 93-100% / 90-100% for EDTA plasma, and 90-102% / 97-101% / 91-98% for heparin plasma. Spike-and-recovery averaged 100% for serum (range 95-103%), 99% for EDTA plasma (range 96-104%), and 100% for heparin plasma (range 95-105%). Kit components are stored at 2-8°C for 12 months, and the associated UniProt entry is O88855.

The protocol proceeds through plate and reagent equilibration, a 90 minute primary incubation at 37°C, a 60 minute biotin-labeled detection antibody step, a 30 minute HRP-Streptavidin (SABC) step, and TMB colour development in the dark for 10-20 minutes, after which stop solution is added and absorbance is read at 450 nm. Sample preparation guidance is provided for serum (clot, centrifuge at 1000 × g for 20 minutes), plasma (anticoagulant tubes, 1000 × g for 15 minutes at 2-8°C), tissue homogenates in PBS with protease inhibitors, cell culture supernatant (2500 rpm, 5 minutes), and cell lysates prepared with protease inhibitors.