Mouse Leptin R PharmaGenie ELISA Kit (SBRS1453)

Quantitative measurement of the mouse leptin receptor (LepRb/OB-R) supports studies of energy homeostasis, appetite regulation, and metabolic disease mechanisms in mouse models. The leptin receptor is a class I cytokine receptor that mediates leptin's central effects on satiety, body weight, and glucose homeostasis, and changes in its circulating levels are reported in obesity, type 2 diabetes, and leptin resistance research.

Assay Genie's Mouse Leptin R PharmaGenie ELISA Kit is configured to specifically detect mouse LepRb in cell culture supernatant, plasma, and serum specimens. The assay is specific to the mouse leptin receptor and is supplied with reagents sufficient for 96 individual determinations using a standard microplate-based protocol. Each kit includes pre-coated plates, detection antibody, recombinant standards, and substrate solution, and is intended for research use only. Store refrigerated upon arrival and protect from light to preserve reagent activity, and avoid repeated freeze-thaw cycles of diluted standards.

Typical workflows include quantification of soluble LepRb levels in obese versus lean mouse cohorts, evaluation of receptor changes in diet-induced obesity models, screening of candidate therapeutics on leptin pathway activity in vitro, and characterization of leptin resistance phenotypes in transgenic or knockout lines. The kit complements downstream applications such as western blot validation, PCR confirmation of LEPR transcripts, and immunohistochemistry of receptor tissue distribution, allowing researchers to triangulate findings across multiple analytical methods.

This Mouse Leptin R ELISA Kit from Assay Genie is intended for laboratory research use only and is not approved for diagnostic procedures.