Mouse Leptin (Lep) ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is specifically designed for the precise quantification of leptin in mouse samples, offering high sensitivity and specificity for research exploring energy balance, obesity, diabetes and other metabolic disorders. The assay is reactive with mouse leptin and is validated for use with serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.

Performance characteristics reported for the assay include a detection range of 0.312-20ng/mL and a sensitivity of 0.165ng/mL. Intra-assay CV is 5.7% and inter-assay CV is 7.9%. Linearity has been assessed in serum, EDTA plasma and heparin plasma across dilutions of 1:2, 1:4, 1:8 and 1:16 (N=5). Average recovery is 87% (range 81-93%) in serum and 89% (range 83-95%) in plasma. The kit recognises both natural and recombinant mouse leptin (UniProt P41160) and is intended for metabolism research.

Leptin is described as a key regulator of energy balance and body weight control, acting in the hypothalamus as an appetite-regulating factor that reduces food intake and increases energy expenditure while also regulating bone mass and hypothalamo-pituitary-adrenal hormone secretion. Peripherally, leptin raises basal metabolism, influences reproductive function, modulates pancreatic beta-cell activity and insulin secretion, and is pro-angiogenic and immunomodulatory. Synonyms include Obesity factor and Ob. For research use only.