Mouse Leptin ELISA Kit

From Assay Genie, this sandwich ELISA kit uses a double-antibody format and a colorimetric detection method to quantify leptin levels in mouse samples. The kit is reactive with mouse specimens and is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, making it suitable for research into metabolic disorders, obesity, energy balance, appetite control, and related pathways in mouse models.

Performance characteristics quoted from the manufacturer include a sensitivity of 37.5 pg/mL and an assay range of 62.5-4000 pg/mL (1ng=1700mIU). Reported linearity across dilutions spans 85-101% (serum), 90-100% (EDTA plasma), and 85-102% (heparin plasma), while recovery averages 93% in serum, 96% in EDTA plasma, and 94% in heparin plasma. The protocol uses a TMB substrate with sequential incubations at 37°C and a final absorbance read at 450 nm. Unopened components are stored at 2-8°C for 12 months. Associated identifiers listed by the manufacturer include the synonym set Leptin, LEP, LEPD, OBS, and Obesity Homolog, and the protein entry P41160.