Mouse LC3B/MAP1LC3B ELISA Kit

A sandwich ELISA, double antibody kit from Assay Genie supports the precise measurement of LC3B levels in mouse samples. LC3B, also known as microtubule-associated protein 1 light chain 3 beta, is a key player in autophagy, a process that maintains cellular homeostasis by degrading damaged organelles and proteins. Dysregulation of autophagy has been linked to cancer, neurodegenerative disorders, and metabolic conditions, making LC3B an important biomarker for studying these diseases and developing potential treatments.

Reported sensitivity is 46.875 pg/mL with a working range of 78.125-5000 pg/mL. Listed sample types are serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Reactivity is mouse and storage is 2-8°C for 12 months.

The workflow uses a biotin-labelled detection antibody and HRP-Streptavidin (SABC), with TMB substrate for colour development and absorbance read at 450 nm after stop solution is added.