Mouse Klk1b22/Kallikrein 1-related peptidase b22 ELISA Kit

Assay Genie supplies a Sandwich ELISA, Double Antibody kit developed for the quantitative detection of mouse Klk1b22 (Kallikrein 1-related peptidase b22). The assay is designed for use with mouse samples and supports a broad range of specimen types including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, making it suitable for diverse research applications.

Performance characteristics include a sensitivity of 46.875 pg/mL and a working range of 78.125–5000 pg/mL. The kit should be stored at 2-8°C for 12 months. Validation data shows linearity across 1:2, 1:4 and 1:8 dilutions, with serum recovery ranging 88-104%, EDTA plasma 82-99%, and heparin plasma 80-100%, while spike-and-recovery averages fall between 96 and 97% across serum, EDTA plasma and heparin plasma. The workflow begins with equilibrating reagents and TMB substrate to room temperature and setting up standards, samples and controls on the pre-coated plate, followed by primary incubation, biotin-labelled detection antibody binding, HRP-Streptavidin binding, TMB colour development in the dark, and a stop step with absorbance read at 450 nm. KLK1B22 is described as an enzyme involved in the regulation of blood pressure and fluid homeostasis, with a recognised role in inflammatory and immune responses.