Mouse Kinase suppressor of Ras 2 (Ksr2) ELISA Kit

Designed for the accurate quantification of KSR2 levels, this Sandwich ELISA Kit from Assay Genie targets the mouse Kinase suppressor of Ras 2 protein using colorimetric detection. The kit is intended for research use only and supports studies of KSR2, a scaffold kinase involved in MEK/RAF signaling that influences cell growth, differentiation, and survival. Dysregulation of KSR2 has been associated with cancer, diabetes, and neurological disorders, making reliable measurement of this target relevant to disease research.

Performance characteristics include a detection range of 0.156–10 ng/mL and a sensitivity of 0.087 ng/mL. Intra-assay CV is 7.8% and inter-assay CV is 10.0%. Linearity has been evaluated in serum, EDTA plasma, and heparin plasma across dilutions of 1:2, 1:4, 1:8, and 1:16 (N=5), with recovery of 82% (range 80–88%) for serum and 84% (range 80–90%) for plasma. The assay detects natural and recombinant mouse Kinase suppressor of Ras 2.

The kit is validated for use with mouse serum, plasma, tissue homogenates, cell culture supernatants, and other biological fluids. Most reagents are stored at -20°C, with wash buffer, substrate, and stop solution stored at 4°C. The associated UniProt entry is Q3UVC0, with a reported molecular weight of 108,572 Da for the target protein.