Mouse  IS (serum indoxyl sulfate) ELISA Kit (MOFI01377)

For research applications involving uremic toxin profiling in rodent models, this quantitative immunoassay enables measurement of indoxyl sulfate, a protein-bound solute that accumulates during chronic kidney disease and has been investigated for its role in vascular dysfunction and tubular injury. The kit, supplied by Assay Genie, is configured for 96 Assays and is intended for use with specimens of murine origin, including serum or plasma fractions. Indoxyl sulfate is a tryptophan-derived metabolite produced by hepatic sulfonation of indole, and quantifying its circulating concentration supports studies of gut-kidney axis signaling, microbiota-derived uremic retention solutes, and progression of renal fibrosis in experimental mouse cohorts.

The assay follows a matched-pair antibody ELISA format with a colorimetric readout, and the included microplate is pre-coated with a capture reagent specific to indoxyl sulfate. Researchers run a standard curve alongside unknowns to interpolate concentrations, and the kit provides sufficient reagents to process one full plate of unknowns, standards, and quality controls in a single run. Working reagents and unused strips should be handled according to the package insert, with reconstituted standards kept refrigerated and protected from light when not in use.

Validated for mouse reactivity, the kit is intended for research use only and is not approved for diagnostic or clinical decision-making. It is commonly ordered by nephrology, cardiovascular, and toxicology laboratories that need to correlate circulating indoxyl sulfate with creatinine, BUN, or histological markers of kidney injury in experimental mouse cohorts.