Mouse Interleukin 5 Receptor Alpha (IL-5Ra) ELISA Kit

Interleukin-5 receptor alpha (IL-5Rα, CD125) is the ligand-binding subunit of the IL-5 receptor, a Th2-associated signaling complex that drives eosinophil maturation, recruitment, and activation. The Assay Genie Mouse IL-5Rα ELISA Kit is a microplate-based immunoassay for quantitative measurement of this receptor in mouse-derived samples. Investigators studying allergic airway inflammation, helminth immunity, and eosinophilic disease models in mice use IL-5Rα as a molecular readout of those pathways.

Each kit includes a capture antibody-coated plate, biotinylated detection antibody, streptavidin-HRP reporter, recombinant mouse IL-5Rα standards for curve generation, wash buffer, substrate, and stop solution. The protocol follows an immunoassay workflow read on a standard microplate reader. Reagents should be kept refrigerated and protected from light to preserve activity; reconstituted standards and samples should be aliquoted to limit freeze-thaw cycles. The full kit is supplied for in vitro research use only and is not intended for diagnostic procedures.

Researchers commonly apply this assay to supernatant from cultured splenocytes, bronchoalveolar lavage fluid collected from mouse asthma models, tissue homogenates, and blood fractions from inflammation studies. Reagents are arranged so each plate supports the indicated number of determinations per protocol, and the kit can be completed within a single working session once standards and samples are prepared.