Mouse Interleukin 22 (IL-22) ELISA Kit

Built by Assay Genie for the quantitative measurement of mouse Interleukin-22, this microplate ELISA kit supports research studies in mucosal immunology, inflammatory disease models, and barrier-tissue host-defense signaling. IL-22 is an alpha-helical cytokine of the IL-10 family produced by activated Th17 cells, Th22 lymphocytes, and group 3 innate lymphoid cells, where it signals through the IL-22R1/IL-10R2 receptor complex on epithelial, hepatic, and keratinocyte lineages.

The assay uses a paired-antibody format with absorbance-based readout, accommodating up to 96 determinations per plate. It is validated across multiple matrices including tissue homogenates, cell lysates, and cell culture supernatant, and can also be applied to standard liquid biospecimens from in vivo mouse work. Researchers studying inflammatory bowel disease, psoriasis, or airway and gut infection models commonly use IL-22 quantification to monitor disease progression, barrier integrity, and therapeutic response.

Each kit is supplied with a pre-coated microplate, detection antibody, recombinant standard, substrate, and buffers sufficient to complete the full protocol. The assay is configured for research use only and is shipped with reagents that should be stored refrigerated, with the standard typically aliquoted to limit freeze-thaw cycles and preserve activity through the kit's stated shelf life.