Mouse Interleukin 12A (IL-12A) ELISA Kit

Quantifying mouse IL-12A (p35) protein in experimental specimens is central to studies of Th1-polarized immunity, and the Assay Genie Mouse Interleukin 12A (IL-12A) ELISA Kit provides the reagent set needed for that work. IL-12A pairs with the p40 subunit (IL-12B) to form the bioactive heterodimer IL-12, a cytokine that drives T-helper 1 differentiation and interferon-gamma production. Because IL-12 sits at the intersection of innate and adaptive immunity, laboratories use quantitative IL-12A readouts to track immune activation in mouse models of infection, inflammation, autoimmunity, and cancer.

The kit is configured as a two-antibody (capture plus detection) immunoassay and is supplied with the reagents and consumables required to process a full plate of mouse samples. The procedure follows a standard add-sample-then-detection-antibody workflow, finishing with a colorimetric read on a conventional plate reader. The plate layout supports a multi-point standard curve plus sample replicates, allowing researchers to interpolate unknowns against a freshly run standard on every plate.

Typical research applications include cytokine profiling in mouse disease models, characterization of vaccine-induced immune responses, monitoring of tumor-associated inflammation, and mechanistic dissection of Toll-like receptor and T-cell receptor signaling pathways that drive IL-12 production. IL-12A measurements are frequently paired with IFN-gamma, IL-4, IL-12B, and IL-18 readouts to map the broader Th1/Th2 balance in mouse experimental systems.

For storage, unopened components should be kept refrigerated and protected from light until first use; reconstituted standards should be aliquoted to limit repeated freeze-thaw cycles. The kit is labeled for research use only and is not validated for clinical diagnostic or therapeutic decision-making.