Mouse Interleukin 11 (IL-11) ELISA Kit

The Mouse Interleukin 11 (IL-11) ELISA Kit from Assay Genie is configured for quantitative measurement of IL-11, a pleiotropic cytokine of the IL-6/gp130 family involved in hematopoiesis, bone metabolism, epithelial repair, and inflammatory signaling. The sandwich immunoassay format pairs pre-coated capture antibodies with biotinylated detection antibodies and streptavidin-HRP, with absorbance recorded at 450 nm. The standard curve covers 15.6 to 1000 pg/mL, with a minimum detectable sensitivity below 9.38 pg/mL. The protocol uses 100 µL of sample per well and an incubation schedule of approximately 3.5 hours at room temperature. Reconstituted standards should be aliquoted and frozen at -20 °C, while unused strip wells and the sealed microplate are stable refrigerated at 2-8 °C. Researchers use this kit to monitor IL-11 secretion in mouse models of pulmonary fibrosis, rheumatoid arthritis, colitis, stromal-driven tumor progression, and stromal-epithelial crosstalk studies, with validated recovery from serum, plasma, tissue homogenates, and cell culture supernatants.