Mouse Interleukin 1 Receptor Associated Kinase 1 (IRAK1) ELISA Kit

As a serine/threonine kinase operating at the MyD88-dependent signaling hub downstream of Toll-like receptors and the IL-1 receptor, IRAK1 recruits TRAF6 and propagates TAK1-mediated NF-κB activation, making it a quantitative marker of innate immune pathway engagement. The Assay Genie Mouse IRAK1 ELISA Kit provides an immunoassay for measuring this kinase in mouse-derived samples, supporting TLR signaling studies, kinase inhibitor screening, and inflammatory disease modeling.

This 96-assay format is validated for mouse reactivity and accepts tissue homogenates and cell lysates as input material, enabling work with cultured macrophage, dendritic, or epithelial cell lines and with tissue extracts from in vivo mouse models. The assay produces an absorbance-based readout compatible with standard microplate readers and is supplied as a complete reagent set sufficient for sample incubation through final measurement.

IRAK1 quantification fits several immunology workflows: profiling pathway activity after TLR ligand stimulation, evaluating candidate IRAK1 or IRAK4 inhibitors in vitro, tracking kinase levels in vivo in sepsis and autoimmune models including rheumatoid arthritis and lupus, and characterizing MyD88-dependent responses in pathogen-defense studies. The kit is configured for research use only and is appropriate for academic laboratories, pharmaceutical discovery groups, and translational programs investigating innate immune regulation.