Mouse Interleukin 1 Receptor Antagonist (IL-1RA) ELISA Kit

Interleukin-1 Receptor Antagonist (IL-1RA) is an endogenous anti-inflammatory cytokine that competitively inhibits IL-1α and IL-1β binding to the type I IL-1 receptor. Quantitative measurement of this mediator supports murine research into rheumatoid arthritis, sepsis, inflammatory bowel disease, and autoimmune disease models. The Assay Genie sandwich-format kit pairs pre-coated capture antibodies with a chromogenic reporter system, allowing researchers to generate a standard curve and quantify endogenous IL-1RA in experimental mouse samples.

The assay accommodates serum, plasma, tissue homogenates, and cell culture supernatants, with a working range of 15.6 to 1000 pg/mL, a minimum detectable dose near 9.4 pg/mL, and a 100 µL sample volume per well. These specifications make it appropriate for profiling cytokine balance, evaluating anti-inflammatory therapeutics, and comparing IL-1 pathway activity between wild-type, knockout, and treated mouse cohorts.

Reagents should be stored at 2-8°C; any unused components may be frozen at -20°C for longer-term retention. The microplate strip format supports running partial plates when sample throughput is limited, and all buffers include preservatives to maintain stability across multiple freeze-thaw cycles.