Mouse Interferon regulatory factor 3 (Irf3) ELISA Kit

From Assay Genie, this high-quality Sandwich ELISA Kit enables quantitative measurement of mouse Interferon Regulatory Factor 3 (IRF3) in serum, plasma, tissue homogenates, cell culture supernatants, and other biological fluids. The kit is reactive specifically with mouse samples.

The assay covers a detection range of 31.2–2000 pg/mL with a sensitivity of 15.6 pg/mL. Precision is supported by an intra-assay CV of 8.3% and an inter-assay CV of 10.3%. Linearity has been characterised at 1:2, 1:4, 1:8, and 1:16 dilutions across serum (98–108%, 108–119%, 86–95%, 101–110%), EDTA plasma (88–97%, 96–105%, 97–106%, 91–102%), and heparin plasma (108–119%, 93–102%, 98–108%, 105–115%). Recovery averages 84% (range 80–90%) for serum and 86% (range 80–92%) for plasma.

IRF3 is a key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. The protein is found in the cytoplasm and nucleus, shuttling between these compartments, and supports research within the immunology field.