Mouse Interferon Beta (IFNb) ELISA Kit

The Assay Genie Mouse Interferon Beta (IFNβ) ELISA Kit is a quantitative two-site immunoassay formatted as a complete microplate kit with 96 assays per box, validated for quantifying endogenous IFN-β across multiple sample matrices from mice, including serum samples, plasma fractions, tissue homogenates, cell lysates, and cell culture supernatant. The procedure uses a pre-coated monoclonal capture antibody paired with a biotinylated detection reagent and produces a colorimetric readout proportional to IFN-β concentration in each well. Typical run time is roughly 3.5 hours from sample loading to final absorbance measurement. The kit ships with lyophilized standards, streptavidin-HRP conjugate, wash buffer concentrate, TMB substrate solution, and stop reagent sufficient for one full microplate. Reconstituted reagents should be kept refrigerated; the standard curve should be aliquoted and frozen for repeated use to limit freeze-thaw cycles, while sealed strip wells may be held at room temperature. This kit is intended for research use only and supports studies of antiviral signaling, viral infection models, and innate immune regulation in mouse systems.