Mouse Intercellular adhesion molecule 3/ICAM3 ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is designed for the precise measurement of Intercellular Adhesion Molecule 3 (ICAM3) levels in mouse samples. With a sensitivity of 0.234 ng/mL and a detection range of 0.391-25 ng/mL, the kit supports research into ICAM3's role in mediating cell adhesion, immune responses, and inflammatory processes. ICAM3 dysregulation has been linked to autoimmune disorders and inflammatory conditions, and the kit enables investigation of disease pathogenesis and potential therapeutic targets in mouse models.

The Mouse ICAM3 ELISA Kit is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. It employs a double antibody sandwich format with colorimetric detection and should be stored at 2-8°C for 12 months. Linearity values at 1:2, 1:4, and 1:8 dilutions (n=5) are 87-99%, 85-105%, and 90-95% for serum; 82-99%, 82-101%, and 82-94% for EDTA plasma; and 81-93%, 81-99%, and 85-100% for heparin plasma. Recovery averages 95% for serum, 97% for EDTA plasma, and 94% for heparin plasma.

The six-step assay procedure includes reagent and plate preparation, primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody incubation at 37°C for 60 minutes, HRP-Streptavidin (SABC) binding at 37°C for 30 minutes, TMB substrate color development in the dark for 10-20 minutes, and stop reaction with absorbance measurement at 450 nm. The associated UniProt entry is Q60625, and the kit is also referenced by synonyms ICAM-3, CD50, CDW50, ICAM-R, and intercellular adhesion molecule 3.