Mouse Integrin alpha-4 (Itga4) ELISA Kit

From Assay Genie, this Sandwich ELISA targets mouse Integrin alpha-4 (Itga4), a protein involved in cell adhesion and migration and implicated in immune responses, tissue regeneration, inflammation, autoimmune disorders, and cancer. The colorimetric assay is specific for natural and recombinant mouse Integrin alpha-4 and is supplied for research use only.

Performance characteristics include a detection range of 78-5000pg/mL, a sensitivity of 39pg/mL, an intra-assay CV of 3.5%, and an inter-assay CV of 8.3%. Dilutional linearity in serum covers 107-118% at 1:2 to 106-116% at 1:16; EDTA plasma covers 97-107% to 104-113%; and heparin plasma covers 103-114% to 104-114% across the same dilution series. Recovery averages 87% for serum (range 81-93%) and 89% for plasma (range 83-95%).

Validated sample types include serum, plasma, tissue homogenates, cell culture supernatants, and other biological fluids. Components include a dismountable microplate, lyophilized standard, sample and assay diluents, detection reagents, wash buffer, substrate, stop solution, and plate sealers, with component-specific storage at -20°C or 4°C. Integrin alpha-4 is listed under synonyms including CD49 antigen-like family member D, Integrin alpha-IV, Lymphocyte Peyer patch adhesion molecules subunit alpha, VLA-4 subunit alpha, LPAM subunit alpha, and CD49d, with UniProt accession Q00651 and a molecular weight of 115,696 Da. Absorbance is read at 450 nm on a standard microplate reader.