Mouse Integrin Alpha 1 (ITGa1) ELISA Kit

The Mouse Integrin Alpha 1 (ITGa1) ELISA Kit from Assay Genie enables quantitative measurement of ITGA1 (also known as CD49a, the alpha subunit of the VLA-1 heterodimer) in mouse-derived specimens. Integrin alpha-1 pairs with the integrin beta-1 subunit to form the VLA-1 receptor, a cell-surface adhesion molecule that binds collagen IV and laminin-1, and is widely investigated in mesenchymal cell migration, T-cell retention in non-lymphoid tissues, and experimental models of organ fibrosis affecting the kidney and liver.

Researchers apply this microplate-format assay to profile ITGA1 expression in mouse models of inflammation, wound healing, tumor microenvironment biology, and mesenchymal stem cell research, where ITGA1 serves as a marker of mesenchymal lineage commitment. The procedure follows a standard ELISA workflow: reconstitute standards, load samples and standards onto pre-coated wells, incubate with biotinylated detection antibody followed by streptavidin-HRP conjugate, develop with chromogenic substrate, and read absorbance. Reagents should be kept refrigerated between uses and protected from light to preserve the activity of the enzymatic conjugate.