Mouse Insulin receptor substrate 2 (Irs2) ELISA Kit

A Sandwich ELISA kit from Assay Genie, this assay is designed for the quantitative measurement of mouse Insulin Receptor Substrate 2 (IRS2), also referred to as 4PS or IRS-2, in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. The kit uses a colorimetric ELISA detection method with reactivity against mouse samples, and specifically recognises natural and recombinant mouse IRS2. It supports research into IRS2's role in insulin signalling and related processes such as glucose metabolism and cell growth, and is supplied for research use only.

Performance characteristics for this kit include a detection range of 0.312–20 ng/mL and a sensitivity of 0.175 ng/mL. Reported precision values are an intra-assay CV of 6.8% and an inter-assay CV of 8.8%. Linearity has been evaluated in serum, EDTA plasma and heparin plasma across serial dilutions. Recovery in serum averages 84% (range 80–90%), while in plasma it averages 86% (range 80–92%).

By UniProt annotation, IRS2 (accession P81122) is an adaptor/scaffold protein that may mediate the control of various cellular processes by insulin, with a molecular weight of 136,763 Da. The listed research area for this target is cancer.