Mouse Insulin receptor substrate 1 (Irs1) ELISA Kit

A sandwich ELISA kit from Assay Genie is designed for the quantitative detection of mouse Insulin Receptor Substrate 1 (IRS1) in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. The kit uses a colorimetric ELISA detection method and is reactive with mouse samples. Synonyms listed for the target include IRS-1 and Irs-1.

Reported performance characteristics include a detection range of 0.312–20 ng/mL and a sensitivity of 0.171 ng/mL. Intra-assay CV is 8.2% and inter-assay CV is 10.1%. Specificity covers natural and recombinant mouse Insulin Receptor Substrate 1. Linearity is reported for serum, EDTA plasma, and heparin plasma across serial dilutions (1:2, 1:4, 1:8, and 1:16), and recovery is given as 80–90% (average 84%) for serum and 80–92% (average 86%) for plasma.

Functionally, IRS1 is an adaptor protein that mediates insulin-controlled cellular processes. When phosphorylated by the insulin receptor, it binds SH2-domain proteins such as the phosphatidylinositol 3-kinase p85 subunit and GRB2, activating phosphatidylinositol 3-kinase. Dysregulation of IRS1 has been associated with metabolic disorders including diabetes and obesity, supporting its value as a biomarker in research on insulin signaling. The kit is for research use only.