Mouse Insulin Promoter Factor 1 (IPF) ELISA Kit

The Mouse Insulin Promoter Factor 1 (IPF) ELISA Kit from Assay Genie quantitatively measures IPF (also known as PDX-1), a pancreatic homeodomain transcription factor required for insulin gene activation and beta-cell identity. The two-site ELISA workflow uses a pre-coated microplate, biotinylated detection antibody, streptavidin-HRP, and a TMB substrate for absorbance-based readout. The kit is supplied with a 100 μL sample volume protocol and shows no significant cross-reactivity with rat or human PDX-1 homologs, supporting clean readouts in mouse-derived tissue homogenates and cell lysates. Reagents should be refrigerated between uses, with the standard and detection antibody protected from repeated freeze-thaw cycles. Intended for research use only, the kit supports studies in diabetes, islet biology, and pancreatic development, including work on MODY4 and beta-cell differentiation pathways.