Mouse Insulin PharmaGenie ELISA Kit (SBRS1448)

This quantitative immunoassay from Assay Genie provides a sensitive means of measuring insulin concentrations across mouse-derived specimens. Researchers investigate pancreatic islet function, peripheral insulin sensitivity, and compensatory secretory responses in metabolic disease models using the assay. Each plate supports 96 individual reactions in a microwell strip configuration, accommodating both small pilot studies and larger screening campaigns.

Validated for cell culture supernatants, plasma, and serum specimens, the assay integrates into workflows spanning primary islet cultures, ex vivo tissue perfusion experiments, and longitudinal in vivo sampling. Reagent components include pre-coated capture plates, detection antibodies, working standards spanning a serial dilution series, and enzyme conjugate ready for immediate use after equilibration to room temperature.

Data acquisition relies on standard absorbance plate reading following substrate development, with quantitative concentration values derived from a parallel standard curve. Applications include screening glucolipotoxic compounds, characterizing transgenic mouse phenotypes, evaluating insulin secretagogue candidates, and profiling islet graft function in transplantation research. The kit is supplied as a complete set of reagents sufficient for 96 determinations in a single-use box intended for research use only.