Mouse Insulin ELISA Kit

Assay Genie offers this sandwich ELISA kit for the quantitative measurement of insulin in mouse samples. The double-antibody format supports research into glucose regulation and metabolic disorders such as diabetes, where insulin serves as a critical biomarker.

The assay is validated for serum, plasma, cell culture supernatant, cell or tissue lysate and other liquid samples. Detection is colorimetric, with absorbance read at 450 nm following TMB substrate development. Reported performance includes a sensitivity of 46.875 pg/mL and a working range of 78.125–5000 pg/mL. Linearity across serum, EDTA plasma and heparin plasma is given between approximately 82% and 104% at 1:2, 1:4 and 1:8 dilutions (n = 5), with spike-recovery averages of 94% (serum), 95% (EDTA plasma) and 93% (heparin plasma).

Reagents are stored at 2-8°C for up to 12 months. The kit includes a pre-coated dismountable microplate, lyophilized standard and biotin-labelled detection antibody, an HRP-streptavidin conjugate, TMB substrate, purified water, sample, antibody and SABC dilution buffers, 25X wash buffer, stop solution, plate sealers and a technical manual.