Mouse INSL5 (Insulin Like Protein 5) ELISA Kit (MOES01198)

The Assay Genie Mouse INSL5 immunoassay targets the preprohormone encoded at the Insl5 locus on mouse chromosome 4 (NCBI Gene ID 23919; UniProt Q9WUG6). The mature peptide is a two-chain A-B heterodimer released by distal colonic L-cells that also secrete peptide YY and glucagon-like peptide-1, and published murine studies show circulating INSL5 climbs with fasting or prolonged caloric restriction and declines with refeeding. Genetic and chemogenetic manipulation of its cognate Gi/o-coupled receptor RXFP4 alters high-fat diet preference, meal patterning, and ventromedial hypothalamic cAMP signaling, providing a rationale for quantitative tracking in metabolic and gut-brain axis cohorts.

From a workflow standpoint, this Assay Genie kit is supplied as a single microplate pack with lyophilized recombinant mouse INSL5 standards, biotinylated detection antibody, streptavidin-HRP, wash buffer concentrate, TMB substrate, and stop solution. Unopened components are shipped refrigerated and should be held refrigerated until first use, while reconstituted standards and detection reagent are best aliquoted into single-use tubes and stored at -20 °C to limit freeze-thaw loss. Reported intra-assay precision is below 8% CV and inter-assay precision below 10% CV, with spiked recovery of 84-97% in mouse serum, 87-95% in EDTA plasma, and 78-90% in heparin plasma.