Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit

The nuclear factor kappa B (NF-κB) signaling pathway is a central regulator of inflammatory and immune responses, and IκBα (Inhibitory Subunit of NF Kappa B Alpha) plays a pivotal role as the primary cytoplasmic inhibitor that retains NF-κB complexes in resting cells. This colorimetric immunoassay from Assay Genie is configured for quantitative measurement of IκBα in mouse samples, including tissue homogenates and cell lysates, supporting research into pathway activation, phosphorylation dynamics, and inhibitor turnover following cellular stimulation.

Supplied as a complete microplate-format kit providing 96 assays per plate, the product is intended for research use only in laboratory settings investigating transcriptional regulation, cytokine signaling, apoptosis, and innate immunity. Mouse reactivity enables studies with murine cell lines, primary cultures, or tissue extracts derived from animal models of inflammation, cancer, or neurodegeneration. Plate-based setup supports mid-throughput screening of experimental conditions, dose-response curves, or time-course experiments tracking IκBα degradation after TNF-α or LPS stimulation.

Storage and handling should follow the manufacturer's recommendations to preserve antibody binding activity and reagent stability across multiple freeze-thaw cycles. Researchers commonly pair this assay with western blot validation, kinase activity profiling, or transfection studies involving IκBα mutants, providing orthogonal confirmation of NF-κB pathway engagement at the protein level when investigating IKK complex function or proteasomal degradation kinetics.