Mouse Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IkBKb) ELISA Kit

The IKKβ (IkBKb) target is a serine/threonine kinase that phosphorylates IκBα, releasing NF-κB for nuclear translocation and driving transcription of pro-inflammatory mediators. Quantifying IkBKb levels in murine samples supports research into chronic inflammation, autoimmune disease models, sepsis, and tumor progression pathways.

This kit from Assay Genie uses a pre-coated microplate with reagents sufficient for one full plate run in duplicate. The workflow involves adding 100 µL of diluted standards or sample per well, followed by a biotinylated detection antibody, streptavidin-HRP conjugate, and TMB substrate. Stop solution produces a measurable color change read on a standard plate reader. Unopened kit components should be refrigerated until first use, and reconstituted standards can be stored frozen and reused across multiple freeze-thaw cycles without significant loss of activity.

Typical assay runtime is roughly 3 to 4 hours performed at room temperature. The kit is intended for research use only and is not approved for diagnostic procedures. Compatible sample inputs include serum or plasma and tissue homogenates or cell lysates, giving flexibility across in vivo and in vitro experimental designs.