Mouse Inhibin Binding Protein/INHBP ELISA Kit

This Sandwich ELISA, Double Antibody assay from Assay Genie is designed for the accurate detection of Mouse Inhibin Binding Protein (INHBP) in mouse samples including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit has a stated sensitivity of 0.469 ng/mL and a measuring range of 0.781-50 ng/mL. INHBP is a protein involved in inhibin binding that plays a role in regulating TGF-beta signaling and influencing cell growth and differentiation, with dysregulation implicated in various disease states, making it a useful biomarker for research.

The workflow involves incubating samples on a pre-coated plate, followed by biotin-labeled detection antibody binding and HRP-Streptavidin (SABC) binding, with color development using TMB substrate and absorbance read at 450 nm. The kit should be stored at 2-8°C for 12 months. Linearity ranges across 1:2, 1:4, and 1:8 dilutions are 96-105%, 91-104%, and 89-102% for serum (n=5); 87-101%, 82-99%, and 90-101% for EDTA plasma (n=5); and 84-98%, 83-98%, and 85-93% for Heparin plasma (n=5). Recovery ranges are 86-102% (average 93%) for serum (n=5), 87-102% (average 92%) for EDTA plasma (n=5), and 86-101% (average 94%) for Heparin plasma (n=5).