Mouse INHBP (Inhibin Binding Protein) CLIA Kit (MOES00385)

Assay performance

The chemiluminescent reaction produces a stable glow measured in relative light units, typically delivering a 10- to 100-fold lower detection limit than colorimetric ELISA. Standard curves are constructed from a seven-point serial dilution spanning roughly 15.6 to 1000 pg/mL of recombinant mouse follistatin; lot-specific ranges should be verified against the enclosed Assay Genie Certificate of Analysis before each run.

Kit components

Each box contains a pre-coated 96-well microplate sealed in a foil pouch with desiccant, lyophilized recombinant standards, biotinylated detection antibody, streptavidin-HRP conjugate, 20× concentrated wash buffer, assay diluent, and substrate solutions A and B. Reconstituted standards should be aliquoted and stored at -20 °C; limit freeze-thaw cycles to two to preserve immunoreactivity.

Applications

The assay supports quantitative measurement of follistatin in mouse serum, plasma (EDTA or heparin), and conditioned media from granulosa cell or hepatocyte cultures. Typical studies cover pituitary-gonadal axis research, PCOS mouse models, exercise physiology, and skeletal muscle hypertrophy, where follistatin antagonizes myostatin and activin A. It is also used for longitudinal metabolic-cage sampling during dietary or pharmacological intervention.

Handling notes

Equilibrate reagents to room temperature before use and avoid foaming during pipetting. Keep plates sealed with desiccant when not in use, and protect from light and moisture. Run samples in duplicate and include a quality-control serum at two concentrations per plate to minimize inter-plate CV. For tissue lysates, normalize total protein to 1–2 mg/mL prior to loading. Intended for research use only.