Mouse Indoleamine-2,3-Dioxygenase (IDO) ELISA Kit

Quantify murine indoleamine-2,3-dioxygenase (IDO) protein levels with this solid-phase immunoassay from Assay Genie, engineered for translational immunology and tryptophan metabolism research. IDO catalyzes the first and rate-limiting step of tryptophan breakdown through the kynurenine pathway, influencing T-cell activity, immune tolerance, and tumor microenvironment immunosuppression, making this kit valuable for studies of cancer immunotherapy mechanisms, autoimmune disease progression, and chronic inflammatory conditions.

The 96-assay microplate format supports multiple experimental conditions, treatment groups, and replicate wells per run. Validated for serum samples, plasma samples, tissue homogenate preparations, and additional sample matrices, the workflow offers flexibility across in vivo animal study designs and ex vivo tissue analyses. Detection relies on a chromogenic substrate reaction read on standard plate readers, with concentration values derived from a standard curve generated from the included calibrators.

Reagents should be stored refrigerated upon arrival and protected from light to preserve antibody activity through the shelf life of the kit. Intended for research use only, the kit supports laboratory workflows including biomarker screening, treatment response monitoring in mouse disease models, and pathway analysis in immune regulation studies. Each kit contains pre-coated plates, detection antibodies, standards, and assay buffers required to perform the full protocol.