Mouse Immunoglobulin M (IgM) ELISA Kit

A sandwich-format ELISA from Assay Genie provides quantitative measurement of mouse Immunoglobulin M (IgM) in serum, plasma, tissue homogenates, cell culture supernatants, and other biological fluids. The kit is colorimetric and is intended for research use only, supporting studies in immunology such as investigations of immune response dynamics, autoimmune conditions, and the role of IgM in health and disease.

Performance characteristics reported by the manufacturer include a detection range of 7.8-500 ng/mL and a sensitivity of 3.91 ng/mL. Precision is stated as an intra-assay CV of 6.8% and an inter-assay CV of 9.4%. Recovery data show average values of 81% for serum (range 80-87%) and 83% for plasma (range 80-89%). Specificity is directed against natural and recombinant mouse Immunoglobulin M.

IgM is a key component of the immune system and plays a crucial role in the body's defense against pathogens. Due to its polymeric nature, IgM possesses high avidity and is particularly effective at complement activation; by itself it is an ineffective opsonin, though it contributes to opsonization by activating complement and causing C3b to bind to the antigen.